Widjiati, - and Z. Faizah, - and N. Darsini, - and V.F. Hendrawan, - and H.N. Karima, - and C. Chotimah, - and S.B. Sumitro, - and Lita Rakhma Yustinasari, - and A.A.M.N. Kasman, - and J.M. Ntoruru, - and Epy Muhammad Luqman, - (2022) Calcium (Ca2+) Expression and Intensity in Cumulus-Oocyte Complex (COCs) in Kacang Goat after Vitrification. Polish Journal of Veterinary Science, 25 (1). pp. 19-26. ISSN 1505-1773

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Abstract

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm

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